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TRIDENT™: An Integrated Three-Pronged Approach for Accurate Microbiome Taxonomic Classification

TRIDENT™ sets a new benchmark for metagenomic analysis — combining the best of modern classification strategies into a unified, intelligent system for high-confidence microbiome profiling. Current metagenomic classification tools generally fall into three categories:Trident 3 GraphicEach of these methods has distinct advantages but also well-known limitations when used alone. Until now, researchers and industry professionals have had to choose between accuracy, speed, and cost — rarely achieving all three at once.

TRIDENT™ changes that.
By seamlessly integrating marker-based profiling, k-mer classification, and genome-level confirmation into a single optimized workflow, TRIDENT™ captures the strengths of each approach while minimizing their individual weaknesses. The result is a new standard for metagenomic precision and reliability.

With TRIDENT™, your results are:

  • More accurate: deeper and more reliable species detection with fewer false positives and negatives
  • Faster: streamlined processing accelerates turnaround for projects of any scale
  • Cost-effective: optimized workflows reduce redundancy and computational overhead

Unlike traditional profilers that rely solely on marker genes or k-mer statistics, TRIDENT™ adds a final genome-level mapping step, enabling precise calculation of coverage, depth, and relative abundance for each detected genome. These additional quantitative measures strengthen confidence in every taxonomic call, filtering out spurious signals and ensuring that all reported organisms are backed by robust genomic evidence.

Our Human Microbiome Sample Base

53,393 Human Microbiome Biosamples from the SPIRE Catalogue

Our MAG Inventory

436,738 Human Microbiome MAGs sourced from SPIRE, GTDB, and cFMD

Our Species Coverage

8,046 Prokaryotic species, incorporating NCBI RefSeq species across all domains

At its foundation, TRIDENT™ leverages the largest-to-date collection of human microbiome metagenome-assembled genomes (MAGs) built from the Searchable Planetary-scale mIcrobiome REsource (SPIRE) and augmented with genomes from the Genome Taxonomy Database (GTDB) and the curated Food Microbiome Database (cFMD), collectively representing one of the largest curated collections of high-quality MAGs for the human microbiome. This expansive, high-quality reference set empowers TRIDENT™ to achieve unparalleled resolution across the diversity of the human microbiome, enhancing both discovery and diagnostic applications.

In short, TRIDENT™ redefines microbiome analysis. Whether your focus is research, product development, or clinical diagnostics, this innovation delivers deeper insights, reduced uncertainty, and greater confidence in your microbiome data — giving you a decisive edge in scientific and commercial advancement.

Study Design

Sample Processing

Sequencing

Compositional and Functional Microbiome Analysis

Publication/Patent Ready Outputs