Different methods for surveying microbial communities provide different answers. We advise our clients on the method of sequencing that is most effective for answering their specific questions. SeqBiome has experience with all of the major platforms and can identify the one best suited to your needs.
We provide 16S rRNA or custom amplicons, shallow and deep shotgun sequencing of metagenomes and metatranscriptomes. Our bioinformatic analysis services cover a broad range of microbiomes, facilitating in-depth analyses of microbial taxonomy (16S rRNA gene/transcript amplicons), functional potential (metagenomics) and activity (metatranscriptomics).
Some studies require an exact list of the microbes present that can be referenced with past work and carried forward to future studies while other studies focus on changes in specific microbial strains or metabolic ability, or what biomolecules a community can produce. We draw on all these approaches to reconstruct how microbes behave and interact in the context of your research.
SeqBiome employs an extensive array of positive controls (mock communities, replicates across runs to ensure consistent results) and negative controls (e.g. to investigate the presence of kit and other contaminants within samples received).