For some questions, the answer is an exact list of the microbes present, that can be referenced with past work and carried forward to future studies. Other questions focus on changes in specific microbial strains or metabolic ability, or what biomolecules a community can produces. We draw on all these approaches to reconstruct how microbes behave and interact in the context of your research.
Different methods for surveying microbial communities provide different answers. We advise our clients on the method of sequencing that is most effective for answering their specific questions. The SeqBiome team have experience in working with multiple Illumina, ION, Oxford Nanopore Technology platforms and can work with you to identify the platform that best suits your needs.
SeqBiome employ an extensive array of positive controls (mock communities, replicates across runs to ensure consistent results) and negative controls (e.g., to investigate the present of kit and other contaminants within samples received).
SeqBiome provide 16S rRNA amplicon, shallow and deep shotgun sequencing of metagenomes and metatranscriptomes. Our bioinformatic analysis services cover a broad range of microbiomes, facilitating in depth analyses of microbial taxonomy (16S rRNA gene/transcript amplicons), functional potential (metagenomics) and activity (metatranscriptomics).